KMID : 0606920160240030320
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Biomolecules & Therapeutics 2016 Volume.24 No. 3 p.320 ~ p.327
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Columbianadin Inhibits Cell Proliferation by Inducing Apoptosis and Necroptosis in HCT116 Colon Cancer Cells
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Kang Ji-In
Hong Ji-Young Choi Jae-Sue Lee Sang-Kook
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Abstract
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Columbianadin (CBN), a natural coumarin from Angelica decursiva (Umbelliferae), is known to have various biological activities including anti-inflammatory and anti-cancer effects. In this study, the anti-proliferative mechanism of actions mediated by CBN was investigated in HCT-116 human colon cancer cells. CBN effectively suppressed the growth of colon cancer cells. Low concentration (up to 25 ¥ìM) of CBN induced apoptosis, and high concentration (50 ¥ìM) of CBN induced necroptosis. The induction of apoptosis by CBN was correlated with the modulation of caspase-9, caspase-3, Bax, Bcl-2, Bim and Bid, and the induction of necroptosis was related with RIP-3, and caspase-8. In addition, CBN induced the accumulation of ROS and imbalance in the intracellular antioxidant enzymes such as SOD-1, SOD-2, catalase and GPx-1. These findings demonstrate that CBN has the potential to be a candidate in the development of anti-cancer agent derived from natural products.
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KEYWORD
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Columbianadin, Colon cancer, Apoptosis, Necroptosis, Oxidative stress
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